FIG 3.
Egr-1-deficient mice displayed impaired proinflammatory cytokine production following P. aeruginosa lung infection. Wild-type (+/+) and Egr-1-deficient (−/−) mice were intranasally infected with 1 × 109 CFU/mouse of P. aeruginosa 8821 for 4 h or 24 h or with an equivalent volume of saline solution as a control (NT). Mice are sacrificed after infection time points. Lung tissues (A, C, G, E, and I) and BALF (B, D, F, H, and J) were collected for detection of IL-1β (A and B), IL-6 (C and D), TNF (E and F), IL-12 (G and H), and IL-17 (I and J) (n = 7 to 9 ± SEM; *, P < 0.05; **, P < 0.01; ****, P < 0.0001).