FIG 7.

Egr-1 deficiency results in impaired NF-κB activation following P. aeruginosa infection in vivo. Wild-type (+/+) and Egr-1-deficient (−/−) mice were intranasally infected with 1 × 10⁹ CFU/mouse of P. aeruginosa 8821 for 4 h or 24 h or with an equivalent volume of saline solution as a control (NT). Mice are sacrificed after infection time points. Nuclear proteins were extracted and subjected to EMSA by incubation with ³²P-labeled NF-κB (A) and NFAT (C) DNA probes. Scan densitometry was performed for analysis of NF-κB (B) and NFAT (D) activation, and data are expressed as fold change versus wild-type untreated lung (n = 6 ± SEM; *, P < 0.05).