FIG 8.

Egr-1 has no effect on IκBα phosphorylation in macrophages in response to P. aeruginosa infection. Wild-type (+/+) and Egr-1-deficient (−/−) BMMs were infected with P. aeruginosa 8821 at an MOI of 10 for 1 h, 2 h, 4 h, or 6 h or were left untreated (NT). Cell lysates were subjected to Western blotting for determining phosphorylated and total levels of IκBα, as well as of actin as a loading control. Blots are representative of three independent experiments (A). Densitometry analysis of phosphorylated IκBα was normalized to total IκBα (B) (n = 3 ± SEM).