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Agonistic (A) and antagonistic (B) activity of AF fractions in ER (200× diluted, red), AR (2× diluted, blue), and DR (2× diluted, green) responsive cell lines. Antagonistic activity is measured as a decrease in luminescence after co-exposure with 4, 150, and 30 pM of E2, DHT, and TCDD, respectively. Results presented as average % of RLUs of positive control ± SD between experiments (n = 3).
