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. Author manuscript; available in PMC: 2020 Feb 11.
Published in final edited form as: J Vis Exp. 2019 Feb 11;(144):10.3791/59096. doi: 10.3791/59096

Figure 2: Representative results of primary acinar cells plated within collagen or basement membrane matrix after adenoviral infection and stimulation with TGF-α.

Figure 2:

Primary acinar cells from a non-transgenic mouse were infected with GFP-adenovirus, embedded in collagen I or basement membrane matrix, and stimulated with or without TGF-α (50 ng/ml). Twenty-four hours post-infection with GFP adenovirus, images were captured to show infection efficiency. At five days post-infection, the images denote the differences in cells stimulated with or without TGF-α in collagen or basement membrane matrix. Duct-like structures are indicated by white arrowheads and scale bars represent 50 μm. Images were obtained using a Zeiss LSM 880 confocal microscope with the EC Plan-Neofluar 10x/0.3 M27 objective.