Materials
| Name of Reagent/ Equipment | Company | Catalog Number |
|---|---|---|
| 37 °C shaking incubator | Thermo Scientific | SHKE4000-7 |
| 5% CO2, 37 °C Incubator | NUAIRE | NU-5500 |
| 50 ml tubes | Falcon | 352070 |
| Absorbent pad, 20” x 24” | Fisherbrand | 1420662 |
| Adenovirus, Ad-GFP | Vector Biolabs | 1060 |
| Aluminum foil | Fisherbrand | 01-213-101 |
| BD Precision Glide Needle 21G x 1/2 1 | Fisher Scientific | 305167 |
| Beaker, 600 mL | Fisherbrand | FB-101-600 |
| Bleach, 8.25% | Clorox | 31009 |
| Cell Recovery Solution 2 | Corning | 354253 |
| Centrifuge | Beckman Coulter | Allegra X-15R Centrifuge |
| Collagenase Type I, Clostridium histolyticum 3 | Sigma | C0130-1G |
| Dexamethasone 4 | Sigma | D1756 |
| Ethanol, 200 proof | Decon Laboratories | 2701 |
| Fetal Bovine Serum | Sigma | F0926-100mL |
| Forceps | Fine Science Tools | 11002-12 |
| Forceps | Fine Science Tools | 91127-12 |
| Glass slide, 8-well | Lab-Tek | 177402 |
| Hank’s Balanced Salt Solution (HBSS), No calcium, No magnesium, No phenol red | Fisher Scientific | SH3048801 |
| Ice bucket, rectangular | Fisher Scientific | 07-210-103 |
| Instant Sealing Sterilization Pouch | Fisherbrand | 01-812-51 |
| LAB GUARD specimen bags (for mouse after dissection) | Minigrip | SBL2X69S |
| Lentiviral Packaging Mix, Virapower | Invitrogen | 44-2050 |
| Matrigel 5 | Corning | 356234 |
| Parafilm 6 | Bemis | PM992 |
| PBS | Fisher Scientific | SH30028.02 |
| Penicillin-Streptomycin | ThermoFisher Scientific | 15140122 |
| Pipet tips, 10 μl | USA Scientific | 1110-3700 |
| Pipet tips, 1000 μl | Olympus Plastics | 24-165RL |
| Pipet tips, 200 μl | USA Scientific | 1111-1700 |
| Pipet-Aid | Drummond | |
| PIPETMAN Classic P10, 1-10 μl | Gilson | F144802 |
| PIPETMAN Classic P1000, 200-1000 μl | Gilson | F123602 |
| PIPETMAN Classic P20, 2-20 μl | Gilson | F123600 |
| PIPETMAN Classic P200, 20-200 μl | Gilson | F123601 |
| Pipettes, 10 ml | Falcon | 357551 |
| Pipettes, 25 ml | Falcon | 357525 |
| Pipettes, 5 ml | Falcon | 357543 |
| Plate, 12-well | Corning Costar | 3513 |
| Plate, 24-well plate | Corning Costar | 3524 |
| Plate, 35 mm | Falcon | 353001 |
| Plate, 6-well | Falcon | 353046 |
| Polybrene 7 | EMD Millipore | TR-1003-G |
| Polypropylene Mesh, 105 μm | Spectrum Labs | 146436 |
| Polypropylene Mesh, 500 μm | Spectrum Labs | 146418 |
| Scissors | Fine Science Tools | 14568-12 |
| Scissors | Fine Science Tools | 91460-11 |
| Sodium Bicarbonate (Fine White Powder) | Fisher Scientific | BP328-500 |
| Sodium Hydroxide | Fisher Scientific | S318-500 |
| Soybean Trypsin Inhibitor 8 | Gibco | 17075029 |
| Spatula | Fisherbrand | 21-401-10 |
| Steriflip 50 ml, 0.22 micron filters | Millipore | SCGP00525 |
| TGF-α | R&D Systems | 239-A-100 |
| Type I Rat Tail Collagen | Corning | 354236 |
| Waymouth MB 752/1 Medium (powder) | Sigma | W1625-10X1L |
| Weigh boat, hexagonal, medium | Fisherbrand | 02-202-101 |
Comments/Description
1
Use as pins for dissection
2
Referred to as ‘basement membrane matrix recovery solution’ in the manuscript.
3
Create a 100 mg/ml solution by dissolving powder in sterile molecular biology grade water, When thawing one aliquot, dilute to 10 mg/ml, filter sterilize and place 1 ml aliquots at −20 °C.
4
Create a 4 mg/ml solution by dissolving powder in methanol, aliquoting and storing at −20 °C.
5
Referred to as ‘basement membrane matrix’ in the manuscript.
6
Referred to as ‘plastic paraffin film’ in the manuscript.
7
Referred to as ‘viral infection enhancer reagent’ in the manuscript.
8
Create a 40 mg/ml solution by dissolving powder in sterile molecular biology grade water, aliquoting and storing at −20 °C.