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. Author manuscript; available in PMC: 2020 Dec 2.
Published in final edited form as: J Am Soc Mass Spectrom. 2019 Dec 2;30(12):2561–2570. doi: 10.1007/s13361-019-02341-0

Figure 1.

Figure 1.

Workflow of expression, affinity purification, and top-down LC-MS analysis of the recombinant PKA C-subunit. a) The plasmid obtained from Addgene organization was transferred into a vector and expressed in E. coli cells. b) The PKA C-subunit was purified using affinity purification. c) The purified samples were subjected to online LC-MS and MS/MS analysis using a Q-TOF mass spectrometer and complemented with offline MS/MS analysis using a FT-ICR mass spectrometer. d) The modifications were characterized based on MS and MS/MS spectra.