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. Author manuscript; available in PMC: 2020 Dec 2.
Published in final edited form as: J Am Soc Mass Spectrom. 2019 Dec 2;30(12):2561–2570. doi: 10.1007/s13361-019-02341-0

Figure 3.

Figure 3.

Identification of five phosphorylation sites at the N-terminal region using ECD. a) ECD fragment ion mapping for the first 45 amino acid residues. b) Representative fragment ions from ECD fragmentation. Five phosphorylation sites, Ser2/Ser3, Ser10, Ser11, Ser18, and Ser30 were confirmed by phosphorylated c ions.