Fig 1. Experiment overview and expression patterns.

A) Mouse and human pluripotent stems cells were exposed to neural differentiation factors to initiate differentiation and then sampled for RNA-sequencing every 4 or 10 minutes for the first 600 minutes. Expression trends along the time course for each gene were fit using a breakpoint model. B) Expression trends are shown for representative genes having patterns classified as not dynamic, immediate dynamic, or delayed dynamic. Immediate dynamic genes had an immediate up- or downregulation segment, whereas delayed dynamic genes had a flat initial segment. Trends were further characterized as having a peak, dip, or as cyclic (multiple peak/dip) (Methods).