Table 2.

Oligonucleotides used in this work.a

Oligonucleotide	Sequence (5′→3′)	T m (°C)	Use
Pm_ins‐F	TAT CTC TAG TAA GGC CTA CCC CTT AGG CTT TAT GCA AGC TTA GGA GGA AAA ACA TAT GCG	60	Insertion of Pm into vector pSEVA237M by site‐directed mutagenesis PCR
Pm_ins‐R	GCC ATT TTT TGC ACT CCT GTA TCC GCT TCT TGC AAT TAA TTA AAG GCA TCA AAT AAA ACG AAA GGC TCA	59
ssr_nia‐F	CGC TAA CGA CGA TAA CTA CGC CCT GGC TGC GTA AAC TAG TCT TGG ACT CCT GTT GAT AGA	60	Insertion of a nia/ssrA tag into pS23·Pm‐GFP by site‐directed mutagenesis PCR
ssr_nia‐R	GCA CGT TCA TCA GCT TGA TGC ACC ACG ACG TTG GAC TCG CCT TTG TAG AGT TCA TCC ATG CCG TGC	57
xylS·pTn7‐F†	ACG TCT TAA UTA AAC GTT CGT AAT CAA GCC ACT T	61	Amplification of xylS to clone into vector pTn7‐M
xylS·pTn7‐R†	AGG ACA CUG CAC TTT ATG CTG GTT ATG C	60
pTn7·xylS‐F†	AGT GTC CUA GGC CGC GGC CGC	63	Amplification of pTn7‐M to insert xylS or xylS/Pm→nia
pTn7·xylS‐R†	ATT AAG ACG UCT TGA CAT AAG CCT GTT CGG TTC	62
pTn7·nia‐F†	ACT CAG GGU ACC CGG GGA TCC TCT AGA	58
nia·pTn7‐R†	ACC CTG AGU GTA AAC AAA TTC CCC ATC AAG A	57	Cloning of xylS/Pm→nia
Gm_check‐F	AGT CAG AGT TAC GGA ATT GTA GG	55	Checking insertion of GmR into the chromosome
Gm_check‐R	ATT AGC TTA CGA CGC TAC ACC C	56
gfp_RBS‐F	AAT CCT AGG CCG CGA CGC ATG TTT AGG AGG AAA AAC ATA TGC GTA AAG GTG AAG AAC TGT	63	Cloning of msfGFP
gfp‐R	AAG ACT AGT CAT TTA TTT GTA GAG TTC ATC CAT G	54
pS1	AGG GCG GCG GAT TTG TCC	60	Check SEVA plasmids cargo
pS2	GCG GCA ACC GAG CGT TC	59

a. Oligonucleotides designed for USER assembly are indicated with a † symbol.