Table 1.
Plasmids used in this work
| Namea | Relevant featuresb | Source or reference |
|---|---|---|
| pEMG | Suicide vector used for deletions in Gram‐negative bacteria; oriT, traJ, lacZα, oriV(R6K); KmR | Martínez‐García and de Lorenzo (2011) |
| pRK600 | Helper plasmid used for conjugation; oriV(ColE1), mob(RK2), tra(RK2); CmR | Kessler et al. (1992) |
| pGNW2 | Derivative of vector pEMG carrying P 14g→msfGFP | This work |
| pGNW4 | Derivative of vector pGNW2; SmR | This work |
| pGNW6 | Derivative of vector pGNW2; GmR | This work |
| pGNW2·ΔnicX | Derivative of vector pGNW2 carrying HRs to delete nicX (PP_3945) in P. putida KT2440 | This work |
| pGNW4·ΔnicX | Derivative of vector pGNW4 carrying HRs to delete nicX (PP_3945) in P. putida KT2440 | This work |
| pGNW6·ΔnicX | Derivative of vector pGNW6 carrying HRs to delete nicX (PP_3945) in P. putida KT2440 | This work |
| pGNW2·ΔaceEF | Derivative of vector pGNW2 carrying HRs to delete aceEF (PP_0338‐PP_0339) in P. putida KT2440 | This work |
| pSEVA2313 | Expression vector; oriV(pBBR1), P EM7; KmR | This work |
| pS2313·R | Derivative of vector pSEVA2313 carrying P EM7→mRFP1 (Campbell et al., 2002); KmR | This work |
| pS2313·B | Derivative of vector pSEVA2313 carrying P EM7→mBFP2 (Subach et al., 2011); KmR | This work |
| pS2313·O | Derivative of vector pSEVA2313 carrying P EM7→mOrange2 (Shaner et al., 2008); KmR | This work |
| pS2313·T | Derivative of vector pSEVA2313 carrying P EM7→mTurquoise2 (Goedhart et al., 2012); KmR | This work |
| pGNW2·LP::P EM7‐mRFP | Derivative of vector pGNW2 carrying P EM7→mRFP1 | This work |
| pGNW2·LPR | Derivative of vector pGNW2 carrying HRs to insert P 14g(BCD2)→ mRFP1 into a landing pad in the chromosome of P. putida KT2440 | This work |
| pGNW2·LPG | Derivative of vector pGNW2 carrying HRs to insert P 14g(BCD2)→ msfGFP into a landing pad in the chromosome of P. putida KT2440 | This work |
| pGNW2·LPB | Derivative of vector pGNW2 carrying HRs to insert P 14g(BCD2)→mBFP2 into a landing pad in the chromosome of P. putida KT2440 | This work |
| pGNW4·LPO | Derivative of vector pGNW4 carrying HRs to insert P 14g(BCD2)→ mOrange2 into a landing pad in the chromosome of P. putida KT2440 | This work |
| pGNW6·LPT | Derivative of vector pGNW6 carrying HRs to insert P 14g(BCD2)→ mTurquoise2 into a landing pad in the chromosome of P. putida KT2440 | This work |
| pSEVA128S | Helper plasmid; oriV(RK2), xylS, Pm→I‐SceI; AmpR | Aparicio et al. (2015) |
| pSEVA228S | Helper plasmid; oriV(RK2), xylS, Pm→I‐SceI; KmR | Aparicio et al. (2015) |
| pSEVA428S | Helper plasmid; oriV(RK2), xylS, Pm→I‐SceI; SmR | Aparicio et al. (2015) |
| pSEVA628S | Helper plasmid; oriV(RK2), xylS, Pm→I‐SceI; GmR | Aparicio et al. (2015) |
| pSEVA1213S | Helper plasmid; oriV(RK2), P EM7→I‐SceI; AmpR | This work |
| pSEVA6213S | Helper plasmid; oriV(RK2), P EM7→I‐SceI; GmR | This work |
| pSEVA448 | Expression vector; oriV(pRO1600/ColE1), xylS, Pm; SmR | Silva‐Rocha et al. (2013) |
| pSEVA421.Cas9tr | Cloning vector; oriV(RK2), cas9, tracrRNA; SmR | Aparicio et al. (2018) |
| pS448·CsR | Derivative of vector pSEVA448 used for CRISPR‐Cas9 counterselection; xylS (cured of BsaI restriction sites), Pm→cas9, P EM7→sgRNA; SmR | This work |
| pS448·CsR_aceEF | Derivative of vector pS448·CsR carrying P EM7→aceF‐targeting sgRNA; SmR | This work |
a. Plasmids can be obtained from Addgene (http://www.addgene.org) with the following deposit numbers: pGNW2 (122086), pGNW4 (122088), pGNW6 (122093), pSEVA1213S (122095), pSEVA6213S (122094) and pS448·CsR (122096).
b. Antibiotic markers: Amp, ampicillin; Cm, chloramphenicol; Km, kanamycin; Sm, streptomycin; and Gm, gentamicin. HRs, homology regions.