Figure 3.

Effect of Mab 4E4 on T3SS gene expression in Pseudomonas aeruginosa. (A) Exogenous addition of Mab 4E4 suppressed the Spd (10 μM)‐induced T3SS expression. The final concentrations of Spd and Mab 4E4 were 10 and 20 μM respectively. The data were the means of three replicates. (B) Immunoblotting detection of T3SS global regulator ExsA and the effector ExoS. Bacterial cultures were grown in minis medium supplemented with NTA and 10 μM Spd, then 20 μM Mab 4E4 and IgG were added respectively. The protein samples from bacteria were separated by 10% SDS‐PAGE electrophoresis. The experiments were repeated for at least three times with similar results.