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. 2018 May 14;13(1):87–96. doi: 10.1111/1751-7915.13279

Figure 4.

Figure 4

Mab 4E4 attenuates the cytotoxicity of Pseudomonas aeruginosa on mammalian cell line A549. (A) Photograph images of A549 cells without infection (CK1); A549 infected by P. aeruginosa PAO1 (CK2); A549 Cells inoculated with PAO1 plus 200 μM Mab 4E4 (4E4) or IgG (IgG); A549 cells infected by PAO1 pre‐treated 2 h with Mab 4E4 (Pre 4E4 + 4E4) or IgG (Pre IgG+IgG). PAO1 MOI = 50, photos were taken under microscope (10 × objective). (B) Effect of 4E4 on cell viability in A549 cell against PAO1 infection. Viability was measured by WST‐1 test. Data are expressed as percentage on internal control for each cell type measured after over 12 h after cell seeding (time 0) and are the mean ± SEM of three replicates. The experiments were repeated for three times. (C) Effect of Mab 4E4 on cell viability in A549 cell against PA14 infection. Cells were treated with Mab 4E4 or control IgG (4E4 200 μM or control IgG 200 μM) in association with PA14 (MOI = 50) for 3 h compared with normal cells without infection and IgG treatment (CK1) and only infected PA14 without IgG treatment (CK2). Photos were taken under microscope (10 × objective). (D) Effect of 4E4 on cell viability in A549 cell against PA14 infection. Cells were treated with different dosages of 4E4 or control IgG in association with PA14 (MOI = 50) compared with normal cell or PA14 infection alone cell. Viability was measured by WST‐1 test. Data are expressed as percentage on internal control for each cell type measured after over 12 h after cell seeding (time 0) and are the mean ± SEM of three replicates. The experiments were repeated for three times.