Examples of markerless recombineering in P. putida.
A. Chromosomal regions of P. putida
KT2440 examined for markerless deletion using the RecET recombineering system. Red, blue, and purple marks indicate regions knocked out using linear donor dsDNAs, donor plasmid, and both of them respectively. This figure includes the results of the previously reported (Choi et al., 2018) and updated genome engineering.
B. Recombinant P. putida strains ΔpvdD::EGFP, ΔpvdD::Flaviolin, and ΔpvdD::Violacein harbouring heterologous biosynthetic genes/clusters of EGFP, flaviolin, and violacein on the chromosome (Table S2) show green, brown, and purple colours of each product respectively. These results have already been reported in our previous paper (Choi et al., 2018), but we took a new picture of engineered strains for better understanding of the protocols in this paper.