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. 2019 Feb 14;13(1):199–209. doi: 10.1111/1751-7915.13374

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© 2019 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effect of HA length on recombineering efficiency.

The number of tetracycline (Tc)‐resistant colonies appeared after recombineering using 1 μg of donor plasmids pTetSac‐ΔpvdD100::Adaptor, pTetSac‐ΔpvdD200::Adaptor, pTetSac‐ΔpvdD400::Adaptor, pTetSac‐ΔpvdD600::Adaptor, pTetSac‐ΔpvdD800::Adaptor, and pTetSac‐ΔpvdD::Adaptor harbouring pairs of 0.1‐, 0.2‐, 0.4‐, 0.6‐, 0.8‐, and 1.0‐kb HAs respectively (Table S1). Successful knockout of the pvdD gene of all eight colonies randomly selected from each knockout experiment – or all colonies for experiments with less than eight colonies – was verified. The values and error bars represent means and standard deviations of colony counts from triplicate experiments, respectively, while all the actual three data points are also shown.