Figure 2.
Polyesterase activities exhibited by Pseudomonas species. The colonies were grown for 24 h at 30°C on LB agar plates supplemented with different substrates: Tributyrin (esterase activity); coconut oil + rhodamine B (mid‐chain‐length hydrolyzing esterase); Impranil® DLN (synthetic polyester polyurethane, polyesterase activity); PCDM n530, polycaprolactone diol (synthetic polyester, polyesterase activity); and polycaprolactone nanoparticles (current standard for polyesterase screens, polyesterase activity). P. putida as an example for a fluorescent Pseudomonad and E. coli as a negative control are indicated by grey letters. The white halo around P. putida relies on the fluorescence of the siderophore pyoverdine and does not indicate clearance of the substrate. All plates were photodocumented under white light, except coconut oil + rhodamine B‐supplemented plates which were exposed to UV light (λ = 254 nm). Shown are exemplary colonies of a set of at least three colonies for each combination on independent plates. Halo formation of the depicted colony is representative for all replicates.