Figure 2.
Long-read analysis of the VHL locus (Case 1). (A) Reads originating from guide RNA CD.Cas9.XWFV6878.AC and (B) reads originating from guide RNA CD.Cas9.XWFV6878.AA. Each read alignment was split into its 5′ and 3′ component; these data can be reconciled using the displayed read ID. MinION reads mapping to the (+) strand are colored gray, and those mapping to the (−) strand are green. For each Illumina read-pair, the read 1 alignment is colored purple and the read 2 alignment is turquoise. ^ denotes a read’s start site. * denotes a read’s end position. Genomic coordinates refer to human reference sequence build hg19.