. Author manuscript; available in PMC: 2020 Jan 4.

Published in final edited form as: Lab Invest. 2019 Jul 4;100(1):135–146. doi: 10.1038/s41374-019-0283-0

Table 2. Sequencing metrics for MinION datasets.

Case number	Total raw reads	Total alignment-ready reads^#	Read length (bp)^#		Read Quality (bp)^#		Mapped reads (%)	Aligned bases	Equivalent genome coverage	Number of reads mapping with target gene^*	Reads ±25bp surrounding cleavage site (fold enrichment)
Case number	Total raw reads	Total alignment-ready reads^#	Mean	Median	Mean	Median	Mapped reads (%)	Aligned bases	Equivalent genome coverage	Number of reads mapping with target gene^*	+ strand guide	- strand guide
1	274,500	27,886	3,248	1,590	8.9	9.2	97.53	88,356,565	0.029×	72	21 (700×)	15 (500×)
2	84,475	80,068	6,067	3,056	9.0	9.4	96.99	471,766,911	0.16×	436	67 (400×)	82 (500×)

Following adapter removal and quality filtering.

Target gene coordinates. VHL: chr3:10,183,319-10,195,354, DMD: chrX:31,137,345-33,357,726.