Table 1. Sequences of primers used for RT-PCR.
Genes | Primer sequences | Product size (bp) |
---|---|---|
Bax | F: AGAAGGATGATCGCAGCTGTG | 198 |
R: AGTCCAATGTCCAGCCCATG | ||
Survivin | F: GCCAGATGACGACCCCATAG | 199 |
R: GGCACAGCGGACTTTCTTTG | ||
Bcl-xl | F: AGGCAGGCGATGAGTTTGAA | 159 |
R: AGAAAGAGGGCCAVAATGCGA | ||
Gapdh | F: ACAGTCAAGGCAGAGAACGG | 235 |
R: GGTTCACGCCCATCACAAAC |
The annealing temperature for all reactions was 60°C. F: forward primer, R: reverse primer.