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. 2019 Dec 13;7:343. doi: 10.3389/fbioe.2019.00343

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Copyright © 2019 Paradiso, Fitzgerald, Yao, Barry, Taraballi, Gonzalez, Conlan and Francis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell proliferation and migration through a 3D-jellyfish collagen scaffold. (A) SKOV.3 and OVCAR.3 cells proliferation rate on 3D jellyfish scaffold from day 2 to 6 assessed through DNA quantification using PicoGreen® dsDNA quantitation assay. (B) SKOV.3 cells were seeded on the top of the scaffold and they migrated and colonized the entire scaffold to the bottom from day 4 to 14. (C) High magnification of SKOV.3 cells directly interacting with collagen as single cells. (D) OVCAR.3 cells were seeded on the top of the scaffold and they migrated and colonized the entire scaffold to the bottom from day 4 to 14. (E) High magnification of OVCAR.3 cells directly interacting with collagen as cluster of cells. Scale bars indicate 100 um.