Table 1.

PCR primer for bisulfite sequencing.

Gene	Accession number	Primer sequences (from 5′ to 3′)	Production size	CpG number
Irs2	NM_001081212	F: 5′-TTTAAGGGTATTTTTGGTTTGG−3′ R: 5′-ACCATTCACTTATCAAATTCCC−3′	301	30
Map2k4	NM_001316367	F: 5′-TGTTTTTTGATTTTTTTTTTGG−3′ R: 5′-AAAAACTTACAACCCCAAAACT−3′	439	7

Irs2, insulin receptor substrate 2; Map2k4, mitogen-activated protein kinase kinase 4.