Figure 14.

Comparison of DHA-responsive genes associated with innate, adaptive, and inflammation immune pathways in lung, kidney, or spleen tissues 13 weeks post instillation with cSiO₂. Gene expression data were obtained using the NanoString PanCancer Immune Profiling gene panel and are shown as log₂ ratios for cSiO₂-exposed mice fed CON, DHA low or DHA high diets calculated with respect to tissue-matched, vehicle-exposed, CON-fed controls (VEH/CON; log₂ ratio = 0). For the innate (A), adaptive (B), and inflammation (C) pathways, heatmaps with unsupervised clustering (Euclidian distance method) by gene depict log₂ expression values for all genes identified as significantly differentially expressed (FDR q < 0.05, 1.5-fold change) in any one of the indicated tissues. The mean log₂ ratio values + SEM for selected genes of interest are also shown. *p < 0.05 for DHA high compared to CON diet (see Supplementary File 2 for test specifications and FDR-corrected q-values).