Figure 8.

Time course of DHA-responsive genes associated with innate and adaptive immune pathways in lung tissues of mice 1, 5, 9, or 13 weeks post instillation with cSiO₂. Gene expression data were obtained using the NanoString PanCancer Immune Profiling gene panel and are shown as log₂ ratios for cSiO₂-exposed mice fed CON, DHA low, or DHA high diets calculated with respect to time-matched, vehicle-exposed, CON-fed controls (VEH/CON; log₂ ratio = 0). For innate (A) or adaptive (B) immune pathways, heatmaps with unsupervised clustering (Euclidian distance method) by gene depict log₂ expression values for all genes identified as significantly differentially expressed (FDR q < 0.05, 1.5-fold change) at any one of the indicated time points. The mean log₂ ratio values ± SEM for selected genes of interest are also shown. *p < 0.05 for DHA high compared to CON diet and ^#p < 0.05 for DHA low compared to CON diet as determined by nSolver statistical analyses (see Supplementary File 2 for test specifications and FDR-corrected q-values). †p < 0.05 for DHA high compared to CON diet and ‡p < 0.05 for DHA low compared to CON diet as determined by non-parametric Kruskal–Wallis test (for Mx1 only).