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. 2019 Dec 20;39(12):BSR20192803. doi: 10.1042/BSR20192803

Figure 4. Alignment of amino acid residues from F7 fraction.

Figure 4

Alignment of the 18 amino acid residues of F7 fraction of 5 kDa obtained by Edman degradation sequencing. Alignment was performed by Clustal Omega, and the sequence obtained was designated IIFF7Ca peptide. The sequence obtained showed 94% similarity to the sequences of Capsicum annuum: Sequence ID: AHI85723.1 Stress-induced protein 18; Sequence ID: AHI85724.1 Stress-induced protein 19; Sequence ID: XP_016579689.1 flower defensin-like from C. annuum; and Sequence ID: ABY66953.1 Thionin-Like. The percentage of identity between the sequences was determined with Needleman-Wunsch Global Align Protein Sequences methods, and the signal peptides were omitted. Q and L forms of peptide correspond to the two different amino acids identified in the same locus, glutamine (Q) and leucine (L), respectively. These amino acid residues were underlined in the sequence. I% – percentage of identity. P% – percentage of positive residues.