Table 4.
The levels of the independent variables for CIPRO and LEVO analysis.
| Factor | Level | |||
|---|---|---|---|---|
| −1 | 0 | 1 | ||
| A | ACN [%] | 1 | 7 | 13 |
| B | MeOH [%] | 17 | 23 | 29 |
| C | TEA [%] | 0.5 | 1.0 | 1.5 |
| D | NaH2PO4 [mM] | 10 | 30 | 50 |
| E | pH | 2 | 3 | 4 |
| F | flow [ml/min] | 0.7 | 1.0 | 1.3 |
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