Figure 4.

Detection of necrotic tubular epithelial cells using a flow cytometer. (A,B) HK-2 cells were treated with varying concentrations of Ang II (10⁻¹⁰–10⁻⁵ M) for 24 h. (C,D) HK-2 cells were exposed to 10⁻⁹ M Ang II for different times. After treatment, HK-2 (upper panel) cells were stained with annexin V-FITC and PI to determine cell necrosis (annexin V⁺/PI⁺ cells) using a flow cytometry assay. The bar chart shows that the ratio of annexin V⁺/PI⁺ cell numbers was highest in the HK-2 cells treated with 10⁻⁹ M Ang II for 24 h.