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. 2019 Dec 19;10:5785. doi: 10.1038/s41467-019-13708-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Immunoblotting to detect ectopically expressed, HA-tagged wild-type (WT) KRAS and PIK3CA, or KRAS or PIK3CA bearing the indicated amino acid substitutions, in immortalized human uterine epithelial cells. Vinculin, loading control. Viability (b) and proliferation (c) of WT immortalized uterine endometrial epithelial cells or those overexpressing the indicated mutations of KRAS or PIK3CA. b Cells were exposed to 1 μM DNG or vehicle (DMSO) for 72 h. Results are the percentage of viable cells relative to vehicle control cells. Data are the mean + SD of six independent experiments, each with three technical replicates per group. *p < 0.05 by Welch’s t-test. c Cells were exposed to 1 μM DNG or vehicle (DMSO) for 48 h. Results are the percentage of BrdU⁺ cells relative to vehicle control cells. Data are the mean + SD of four independent experiments per group without technical replicates. *p < 0.05 by Welch’s t-test. d Quantitative RT–PCR determination of mRNA levels of PR-A/B in the immortalized cells in a. Values were normalized to GAPDH. Data are the mean fold change + SD relative to levels in control cells stably infected with empty vector. Six independent experiments, each with three technical replicates per group, were conducted. *p < 0.05 by Welch’s t-test. Source data are provided as a Source Data file.