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. 2019 Dec 19;10:5792. doi: 10.1038/s41467-019-13718-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Co-immunoprecipitation of endogenous p62 with exogenously expressed acetyltransferases from HEK293T cells. Acetyltransferases were immunoprecipitated by anti-HA or anti-Flag and the precipitates were analyzed using anti-p62. b Co-precipitation of endogenous TIP60 with p62 from HEK293T cells. c Co-immunoprecipitation of p62 mutants with HA-TIP60 in HEK293T cells. HA-TIP60 was immunoprecipitated by anti-HA and the precipitates were analyzed using anti-Flag. d HEK293T cell lysates were incubated with purified GST-p62-D69A or GST-LC3B, and bound TIP60 and ATG7 were detected by western blot using anti-TIP60 and anti-ATG7. e, f Co-precipitation of Flag-tagged (e) or GFP-tagged (f) p62 truncated mutants with HA-TIP60 from HEK293T cells. HA-TIP60 precipitates were analyzed using anti-Flag and anti-GFP, respectively. Source data are provided as Source Data file.