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. 2019 Dec 19;10:5792. doi: 10.1038/s41467-019-13718-w

Fig. 7. Acetylation enhances p62 body formation.

Fig. 7

a Acetylation of p62 affects the formation of p62 bodies. p62-KO HEK293 cells stably expressing Flag-tagged WT p62, p62-2KQ or p62-2KR were treated with or without starvation, then the cells were stained with anti-Flag and imaged by confocal microscopy. Scale bars, 5 µm. b Quantification of p62 puncta in (a). The data are presented as mean ± S.E.M., n = 30 cells. Two-tailed t test, ***p < 0.001. c In vitro phase separation of p62. Purified recombinant mCherry-tagged WT p62 or each of the indicated p62 mutant proteins was incubated with linear 8 × ubiquitin and visualized by confocal microscopy at different time points. mCherry-p62, 5 μM; linear 8 × ubiquitin, 1.5 μM. Scale bars, 5 µm. d Quantification of the number of mCherry-p62 clusters in c. The data are presented as mean ± S.E.M., n = 5 fields. Two-tailed t-test, *p < 0.05. Source data are provided as a Source Data file.