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. 2019 Dec 19;9:19443. doi: 10.1038/s41598-019-55924-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Average percent of cells which bind Annexin V Alexa Fluor 647 was also studied after shear stress in microfluidic channels. (a) Similar to the FC detection of IgG presence, presence of PS on RBCs was determined using first gates for CD235a FITC fluorescence and on event size by FSC-SSC. (b) The fraction of red blood cells with externalized PS as determined by binding of fluorescently labeled Annexin V was small and did not increase with duration of shear (n = 5). (c) The average fluorescence of the control flow cytometry results was found. That value was then taken as a reference for the relative increase in fluorescence recorded by flow cytometry. The amount of PS fluorescence in the positive subpopulation did increase significantly with exposure time(n = 5, p = 0.01).