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. 2019 Dec 19;10:5794. doi: 10.1038/s41467-019-13621-4

Table 1.

Construction and characterization of the iMAGIC plasmid library.

	LibA	LibI	LibD
CRISPR protein	dLbCas12a-VP	dSpCas9-RD1152	SaCas9
Length of gRNA^a	20 + 23 bp	20 + 82 bp	121 + 127 bp
No. of guides	37,817	37,870	24,806
Fold coverage^b	~133×	~106×	~121×
Mapping ratio	~87.7%	~86.8%	~72.6%
gRNA coverage	~99.9%	100%	~88.9%
Gene coverage^c	100%	100%	~98.3%

^aThe length of guide (underlined) and structural sequences

^bCalculated as estimated library size/number of guide sequences

^cAt least one guide for each gene