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. 2019 Dec 19;9:19474. doi: 10.1038/s41598-019-55411-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overview of the comprehensive analysis strategy for the evaluation of biomaterials in bone regenerative therapy. The natural or synthetic biomaterial is incubated on the CAM of the chicken egg. The neo-vascularization of the scaffold is examined by MRI for its functionality, by MicroCT for the vascular architecture and by histology for the vascular pattern. Together, these quantitative results provide a comprehensive assessment of the ingrown blood vessels. The methods used are presented in tabular form and an in-depth evaluation of the individual layers of the scaffold as well as the advantages and technical limitations of each method are listed.