Fig. 6.

(a) Confirmation of increased NPRC expression by PCR and immunofluorescence in transfected H9C2 cells. (B) Transfected H9C2 cells retain their ability to differentiate based on troponin stain. (c and d) Overexpression of NPRC in H9C2 causes increased cell hypertrophy based on increased cell size. (e) Increased expression of hypertrophic markers, MYH7 and NPPA in NPRC overexpressing H9C2 cells. *p < 0.05,**p < 0.01, and ***p < 0.001 compared to control. Data presented as either mean ± standard deviation or Tukey box-and-whisker plot.

NPRC: natriuretic peptide clearance receptor C.