Fig 6.

Rapamycin is retained in CD4+ T cells. Graph depicting the concentration of Rapamycin in RPMI medium, supernatant and cell pellet at multiple times during 48 h. Medium was stored at 4°C or incubated at 37°C with or without the addition of MACS-sorted CD4+ T cells. The concentration of Rapamycin was measured by LC-MS/MS. (a) Rapamycin concentration in 4°C medium, 37°C medium (n = 4) or supernatant (n = 8) measured five times during 48 h. (b) Rapamycin concentration in CD4+ T-cell pellets (n = 5) measured three times during 24 h.