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. 2019 Sep 17;28(12):1674–1685. doi: 10.1177/0963689719874048

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 6. — EPC transplantation mitigated the ventilation-induced apoptotic damage in lung tissue. The representative results of apoptosis in lung tissue in the S group (Aa), V group (Ab), and EPC transplantation (VE group; Ac) were detected by TUNEL staining using fluorescence microscopy (A, 400 × magnification). TUNEL-positive cells were stained green and the nucleus was stained blue with DAPI. The representative results of the protein expression of Bax (first panel of Ba and Bb), Bcl-2 (second panel of Ba and Bc), native caspase-3 (third panel of Ba and Bd), and cleaved caspase-3 (fourth panel of Ba and Be) in lung tissue in the S group (left bands of Ba, ), V group (middle bands of Ba; ) and VE group (right bands of Ba; ) were detected by Western blot. The expression of the corresponding proteins was evaluated by densitometry analysis, and presented in Bb (Bax), Bc (Bcl-2), Bd (native caspase 3), and Be (cleaved caspase 3). Data in each group were calculated from three independent tests. *p < 0.05 vs. the S group; ^# p < 0.05 vs. the V group. Abbreviations: EPCs, endothelial progenitor cells; S group, sham; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; V group, VILI; VE group, VILI with EPC transplantation; VILI, ventilator-induced lung injury.

Inline graphic — EPC transplantation mitigated the ventilation-induced apoptotic damage in lung tissue. The representative results of apoptosis in lung tissue in the S group (Aa), V group (Ab), and EPC transplantation (VE group; Ac) were detected by TUNEL staining using fluorescence microscopy (A, 400 × magnification). TUNEL-positive cells were stained green and the nucleus was stained blue with DAPI. The representative results of the protein expression of Bax (first panel of Ba and Bb), Bcl-2 (second panel of Ba and Bc), native caspase-3 (third panel of Ba and Bd), and cleaved caspase-3 (fourth panel of Ba and Be) in lung tissue in the S group (left bands of Ba, ), V group (middle bands of Ba; ) and VE group (right bands of Ba; ) were detected by Western blot. The expression of the corresponding proteins was evaluated by densitometry analysis, and presented in Bb (Bax), Bc (Bcl-2), Bd (native caspase 3), and Be (cleaved caspase 3). Data in each group were calculated from three independent tests. *p < 0.05 vs. the S group; ^# p < 0.05 vs. the V group. Abbreviations: EPCs, endothelial progenitor cells; S group, sham; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; V group, VILI; VE group, VILI with EPC transplantation; VILI, ventilator-induced lung injury.