Figure 4.

Comparison of antibacterial effects of MCFAs between the TC and CC. LA (A) and CA (B) were added at 0.25, 0.75, and 2.5 mM in both complexes, and incubated at 37°C for 12 h. Thereafter, the complex was mixed gently and S. aureus (0.3–1 × 10⁵ CFU) was added to the top layer portion. After incubation at 37°C for 12 h, the number of bacterial colonies in the top layer portion was counted with the colony counting method. Data are presented as mean ± SEM of six samples (two independent experiments performed in triplicate). *p < 0.05 compared with the white-colored bar data at LA 0; ^† p < 0.05 compared with the black-colored bar data at LA 0; n.s. = not significant.