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. Author manuscript; available in PMC: 2020 Jun 9.
Published in final edited form as: Nat Immunol. 2019 Dec 9;21(1):54–64. doi: 10.1038/s41590-019-0550-7

Extended Data 3. Loss of RIPK1 sensitizes neutrophils to TNF-mediated cell death.

Extended Data 3.

a-b) Live-cell imaging of CTG-labeled wild-type, Ripk1ΔPMN, and Ripk1D138N/D138N neutrophils treated with 2 μM BPT and/or 10 μM z-VAD-fmk +/− 100 ng/mL TNFα. PI and Annexin V were used to monitor changes in viability. Mean and SEM, n=3 biologically independent samples, and triplicate fields of view per independent biological sample. c) Live-cell imaging of CTG-labeled wild-type, Ptpn6ΔPMN, Ripk1ΔPMN, and Ptpn6ΔPMNRipk1ΔPMN neutrophils treated with saline or 100 ng/mL TNFα. PI and Annexin V were used to monitor changes in viability. Mean and SEM of technical replicates shown. Data are representative of two independent experiments.