Fig. 6.

CD138 surface expression is reversibly controlled by lipoproteins in serum. Freshly isolated myeloma cells were co-stained with PE anti-CD138 and BV510 anti-CD138, then serum starved 1 h and analyzed by flow cytometry. a Representative dot plots comparing PE and BV510 fluorescence before and after starvation, quantifying fluorescence intensity (geometric mean) for both fluorophores with FMO control, representative of two independent experiments. b Serum-starved myeloma recovers CD138 expression following re-culturing for 2 h with 10% FBS containing media, or other recovery conditions. c Human CD138 re-expression following starvation and serum recovery. Replicates pooled from independent experiments and analyzed by t tests. ^*p < 0.05, ^***p < 0.001