Skip to main content
. 2019 Jun 20;77(2):351–363. doi: 10.1007/s00018-019-03185-3

Fig. 5.

Fig. 5

miR-4295 inhibitor reversed ISO inhibition of USP28 and CD44 expression, USP28 3′UTR activity, and cell sphere formation, as well as cell invasion in human BC cells. a miR-4295 inhibitor constructs were stably transfected into T24T cells. The inhibition efficiency of miR-4295 was assessed by quantitative real-time PCR. *Significant difference (p < 0.05). b, c miR-4295 inhibitor could not block USP28 3′UTR luciferase activity and inhibit USP28 and CD44 expression (assessed via Western blot). d, e miR-4295 inhibitor reversed ISO inhibition of cell sphere formation. *Significant difference in comparison to vehicle control (p < 0.05). f miR-4295 inhibitor also reversed ISO inhibition of cell invasion. *Significant difference in comparison to vehicle control (p < 0.05)