Fig. 6.

ISO accelerated miR-4295 maturation by induction of Dicer. a miR-4295 and pre-miR-4295 assessed (via qRT-PCR) in T24T cells treated with 20 μM ISO for the time periods indicated. *Significant difference (p < 0.05). b Dicer expression induced by ISO (20 μM) was evaluated using Western blot. c T24T(vector) and T24T(shDicer) cells were treated with 20 μM ISO for the time periods indicated, and the presence of indicated proteins evaluated using Western blot. d Knockdown Dicer reduced miR-4295 expression using qRT-PCR. *Significant difference in comparison to vector (p < 0.05). e Cell sphere formation by cells transfected with shDicer or nonsense vector. Transfectants were exposed to 20 μM ISO for 7 days before analyses using microscopy. *Significant difference between indicated transfectants and between vehicle- and ISO-treated groups (p < 0.05). f The schematic summary of molecular mechanisms underlying inhibition of human bladder cancer stem cell-like phenotypes and invasivity upon ISO treatment