Fig. 6.

Comparison of the inhibition of let-7a-1 reporter activity mediated by the three let-7a-1 expression systems. a Construction of the let-7a-1 GLuc reporter using the human LIN28B 3′-UTR that contains a let-7a-1-binding site. A mutant-binding site reporter (Mut 3′-UTR) was also constructed as a control. b Subconfluent HEK-293 (a) and HCT116 (b) cells were cotransfected with different combinations of reporter vectors and let-7a-1 expression plasmids. GLuc activity was assessed at 72 h after transfection. Assays were done in triplicate. **p < 0.01 compared with the GLuc-LIN28B + vector control (mock) group