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. 2019 Dec 13;10:841. doi: 10.3389/fendo.2019.00841

Figure 7.

Figure 7

Leptin induces adipogenesis in ASCs independently on insulin. ASCs from subcutaneous and retroperitoneal depots were isolated and then differentiated up to 5 or 12 days with neither insulin nor leptin, leptin (4 or 40 nM), or insulin. (A) Fluorescence images of ASCs differentiated for 12 days and stained with Bodipy (green) for lipid droplets and with DAPI (blue) for nuclei. Images are representative of 3–6 independent experiments from different animal pools. Scale bar represents 50 μm. (B,C) Quantification of the green area per cell in adipocytes differentiated from (B) subcutaneous or (C) retroperitoneal ASCs. Graphs depict the fold change relative to the group with no hormone supplementation at day 5. Bars represent mean ± standard error of the mean of 3–5 independent experiments. *Means p < 0.05 and & means p = 0.06 relative to the control group at the same day. Statistical analyzes were performed by using One-way ANOVA with Dunnet's post-test. (D) Western blot analysis of PLIN1, PPARγ, cleaved SREBP1C (cSREBP1C), and β-actin in subcutaneous ASCs or (E) CAV-1, cSREBP1C, PPARγ, and β-actin in retroperitoneal ASCs after 12 days of differentiation in each condition. Blots are representative of 3–5 independent experiments.