TABLE 3.
Distribution of ppGal and GalR1 mRNA in the mouse brainstem.
Brainstem region | GalR1 | ppGal |
Sp5 | ++ | ++ |
cNTS | +++ | +++ |
rNTS | +++ | − |
XII | ++ | − |
X | + | +++ |
IO | − | ++ |
VRC | + | − |
A1/C1 | + | − |
RTN | + | +++ |
na | + | − |
Reticular nucleus | + | ++ |
Caudal raphe nuclei | ++ | − |
Lateral to A5 | n/a | ++ |
LC | + | +++ |
PbN | +++ | − |
cKF | + | − |
rKF | + | + |
A4 | + | ++ |
Adult male C57BL6/J mice (n = 5) were examined using DIG-incorporated riboprobes complementary to mouse ppGal and GalR1 mRNA by ISH. All anatomical areas listed are according to the atlas of Franklin and Paxinos (Paxinos and Franklin, 2004). −, not expressed; +, scattered sparsely; ++, expressed by 1/3 of the neurons in the area; +++, expressed by > 1/3 of the neurons in the area; n/a, not available.