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. 2019 Dec 20;20(Suppl 24):679. doi: 10.1186/s12859-019-3247-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Bar Plot of Normalization Methods and their relative errors from a two-way ANOVA. The MSE for each of the features (site and biological condition) can be used to measure the amount of variance attributed to that specific feature. The top narrow striped bar is site dependent variability (batch effects); the solid bar is biological variability; and the bottom, wide striped bar is the residual variability