Table 1.
qPCR validation for immunoregulatory/neuroprotective genes
| Gene | qPCR validation fold change (p-value versus control) |
Microarray fold change |
Primer sequence | ||
|---|---|---|---|---|---|
| FGF2 | F2 V2 | FGF2 | F2 V2 | ||
| Cd93 | 240.2 ± 101.2 (***) | 44.9 ± 9.1 (***) | 74.1 | 23.8 | CATCTCACTCTTGCTGGCTCT TCTCCTCTTTCTTGGCTTTCC |
| Il11 | 48.4 ± 19.8 (***) | 14.9 ± 3.7 (**) | 47.5 | 6.3 | CTCCCCTCGAGTGTCTTCAG CCATCAGCTGGGAATTTGTC |
| Hb-egf | 22.2 ± 12.0 (***) | 7.1 ± 2.5 (**) | 11.9 | 4.9 | TTTCTCCTCCAAGCCACAAG TTCCTCTTCTTTTTCCCGTTC |
| Lif | 13.6 ± 4.6 (***) | 3.8 ± 1.7 (*) | 19.4 | 4.0 | CCTTCCCATCACCCCTGT CGTTGAGTTGAGCCAGTTGA |
| Cxcl1 | 12.5 ± 4.1 (***) | 8.4 ± 1.8 (***) | 4.0 | 5.6 | AACCGAAGTCATAGCCACACTC CACCCTTTAGCATCTTTTGGAC |
| Timp1 | 6.2 ± 0.9 (**) | 5.8 ± 1.8 (**) | 2.8 | 2.3 | CTGGTTCCCTGGCATAATCT ATCGCTCTGGTAGCCCTTCT |
| Mmp13 | 28.6 ± 14.0 (***) | 2.2 ± 1.8 (ns) | 28.1 | 1.2 | CTGCGGTTCACTTTGAGGA GAGGCGGGGATAGTCTTTGT |
| Mmp3 | 8.1 ± 3.5 (*) | 0.4 ± 1.2 (ns) | 6.8 | −1.0 | CCCGTTTCCATCTCTCTCAA GACATCAGGGGATTCTGTGG |
Shown are mean +/− SEM of at least 5 independent experiments; p-values for ΔCt of Control vs FGF2 or F2 V2 respectively (one-way ANOVA, Holm-Sidak post-test); * p < 0.05, ** p < 0.01, *** p < 0.001, ns - not significant