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. 2019 Dec 19;16:160. doi: 10.1186/s12985-019-1270-1

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — EOS physically interacts with Tax but not HBZ. Physical interactions between EOS and HTLV-1 regulatory factors (i.e., Tax or HBZ) were investigated by co-immunoprecipitation. The input samples (3%, lanes 1 to 4) and resin-bound proteins (lanes 5 to 8) were subjected to SDS-PAGE followed by western blot analysis. A. Western blot analysis performed on immunoprecipitated EOS protein (EOS-FLAG) revealed a weak but specific physical interaction between EOS and Tax. B. In contrast, no such interaction was demonstrated between EOS and HBZ.