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Cq values of quantitative PCR Plate (v)
| Oocyst quantity | TR1 | TR2 |
|---|---|---|
| 1,000,000 | 29.29 | 28 |
| Mean TR ± SD | 28.65 ± 0.65 | |
| 100,000 | 31.52 | 31.31 |
| Mean TR ± SD | 31.42 ± 0.11 | |
| 10,000 | 34.16 | 34.53 |
| Mean TR ± SD | 34.35 ± 0.19 | |
| 1000 | 37.11 | 37.49 |
| Mean TR ± SD | 37.3 ± 0.20 | |
| 100 | na | na |
| Mean TR ± SD | na | |
| MQ | na | na |
| Mean TR ± SD | na |
Notes: Pure oocyst serial dilution A. Baseline threshold 11.89 RFU
Abbreviations: TR, technical repeat; SD, standard deviation; na, no DNA amplification
