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Cq values of quantitative PCR Plate (v)
| Template dilution | TR1 | TR2 |
|---|---|---|
| ×10 | 32.36 | 31.64 |
| Mean TR ± SD | 32 ± 0.36 | |
| ×100 | 34.84 | 34.95 |
| Mean TR ± SD | 34.9 ± 0.06 | |
| ×1000 | na | na |
| Mean TR ± SD | na | |
| ×10,000 | na | na |
| Mean TR ± SD | na |
Notes: DNA template dilution of 106 oocyst suspension A. Baseline threshold 11.89 RFU
Abbreviations: TR, technical repeat; SD, standard deviation; na, no DNA amplification
