Table 4.
Cq values of Quantitative PCR Plate (vi)
| Oocyst inoculate | Well 1 | Well 2 | Well 3 | ||||
|---|---|---|---|---|---|---|---|
| TR1 | TR2 | TR1 | TR2 | TR1 | TR2 | ||
| 100,000 | 30.25 | 29.52 | 28.29 | 28.83 | 28.4 | 28.98 | |
| Mean ± SD | TR | 28.89 ± 0.37 | 29.06 ± 0.23 | 28.63 ± 0.29 | |||
| BR | 29.21 ± 0.50 | ||||||
| 10,000 | 30.24 | 30.90 | 30.29 | 30.39 | 30.95 | 31.19 | |
| Mean ± SD | TR | 30.57 ± 0.33 | 30.34 ± 0.05 | 31.07 ± 0.12 | |||
| BR | 30.66 ± 0.30 | ||||||
| 1000 | 36.66 | 36.09 | 34.78 | 33.82 | 34.58 | 35.02 | |
| Mean ± SD | TR | 36.38 ± 0.29 | 34.3 ± 0.48 | 34.8 ± 0.22 | |||
| BR | 35.16 ± 0.90 | ||||||
| 100 | 35.83 | 36.06 | 37.6 | 37.73 | 37.04 | 38.64 | |
| Mean ± SD | TR | 35.95 ± 0.12 | 37.67 ± 0.07 | 37.84 ± 0.80 | |||
| BR | 37.15 ± 0.86 | ||||||
| Blank wells | na | 42.80 | 44 | 41.28 | 39.49 | na | |
| Mean ± SD | TR | na | 42.64 ± 1.36 | na | |||
| BR | 41.9 ± 1.69 | ||||||
| Inactivated oocysts | 37.23 | 37.46 | 37.7 | 38.23 | 36.63 | 37.67 | |
| Mean ± SD | TR | 37.35 ± 0.12 | 37.97 ± 0.27 | 37.15 ± 0.52 | |||
| BR | 37.49 ± 0.35 | ||||||
Notes: C. parvum oocysts inoculated onto HCT-8 cell monolayer plate B. Two technical repeats for each well (biological repeat). Baseline threshold 19.88 RFU
Abbreviations: TR, technical repeat; BR, biological repeat; SD, standard deviation; na, no DNA amplification