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. 2019 Nov 19;19(1):460–468. doi: 10.3892/ol.2019.11111

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Exogenous c-myc affects the viability of WERI-Rb1 cells. (A) Morphological changes in WERI-Rb1 cells treated with TSA (250 nM) for 72 h. (B) CCK-8 assays indicated that TSA significantly decreased the viability of WERI-Rb1 cells compared with the negative controls. **P<0.01. (C) Western blotting showing the expression of c-Myc in WERI-Rb1 cells after transfection. (D) Relative expression of c-Myc in WERI-Rb1 cells was quantified using densitometry; the data are presented as histograms. *P<0.05 vs. vector. (E) CCK-8 assays indicated that the viability of WERI-Rb1 cells was reduced following c-myc transfection, compared with the controls. All results were confirmed in triplicate. *P<0.05. TSA, trichostatin A; CCK-8, Cell Counting Kit-8.